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Authors
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Pedziwiatr-Werbicka, E. ; Gorzkiewicz, M. ; Michlewska, S. ; Ionov, M. ; Shcharbin, D. ; Klajnert-Maculewicz, B. ; Pena-Gonzalez, C. ; Sanchez-Nieves, J. ; Gomez, R. ; de la Mata, J. F. ; Bryszewska, M.
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Title
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Evaluation of dendronized gold nanoparticles as siRNAs carriers into cancer cells
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Date
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15.02.2021
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Number
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59958
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Abstract
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Gene therapy is one of the most promising approaches for potential application in the treatment of diseases, ranging from cancer and heritable disorders to infectious diseases. Before nucleic acid molecules can reach their site of action inside target cells, they must overcome several obstacles. Thus, to fully exploit the therapeutic potential of nucleic acids, efficient delivery systems are required. We herein evaluated gold nanoparticles (AuNPs) covered with cationic carbosilane dendrons as siRNA delivery systems. Detailed analysis of formation of AuNP:siRNA complexes using circular dichroism, zeta-potential, zeta-size, electron microscopy and gel electrophoresis was performed. The stability of complexes in presence of heparin and RNase was evaluated. Internalization of AuNPs and their complexes with siRNAs into cancer cells was estimated by ultrastructure analysis and confocal microscopy. The cytotoxicity of dendrons, AuNPs and their complexes with siRNAs on 4 cancer cell lines (Caco-2, HeLa, U937 and THP-1) was estimated. We concluded that dendronization of AuNPs is a promising way to develop siRNA carriers for anticancer gene therapy.
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Publisher
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Journal of Molecular Liquids
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Wikidata
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Citation
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Journal of Molecular Liquids 324 (2021) 114726
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DOI
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https://doi.org/10.1016/J.MOLLIQ.2020.114726
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Tags
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