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Authors
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Abbate, R. A. ; Alkhalaf, M. ; Boye, S. ; Lederer, A.
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Title
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The effect of carrier liquid composition on the molecular properties of caseinate solutions studied by AF4
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Date
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01.03.2020
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Number
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59025
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Abstract
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The effect of carrier liquid composition on the molecular properties of uncross-linked and enzymatically cross-linked caseinate solutions was studied by asymmetrical flow field-flow fractionation (AF4). The sample solutions were prepared in 0.1M NaCl solution and subjected to AF4 analysis using three different elution buffers: 0.1 M NaCl, 0.01 M NaCl, and 0.01M CaCl2. When using 0.1 M NaCl as the carrier liquid, AF4 enabled the separation of the main casein aggregates from larger species (probably residual phospholipids) according to their molar masses, which increase smoothly over the elution times. In contrast, a decrease in the ionic strength of the carrier liquid caused significant changes in the elution properties of the caseinate solutions as a result of variations in the molecular conformations of the aggregates. Our findings extend the knowledge on the molecular properties of complex caseinate solutions as well as point out potential challenges when analyzing dynamic natural systems with an AF4 method.
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Publisher
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LCGC Europe : solutions for separation scientists
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Wikidata
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Citation
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LCGC Europe : solutions for separation scientists 33 (2020) 112–121
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DOI
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https://www.chromatographyonline.com/view/effect-carrier-liquid-composition-molecular-properties-caseinate-solutions-studied-af4
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