Authors
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Rumschöttel, J. ; Baus, S. ; Kosmella, S. ; Appelhans, D. ; Koetz, J.
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Title
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Incorporation of DNA/PEI polyplexes into gelatin/chitosan hydrogel scaffolds: a µ-DSC study
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Date
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28.03.2017
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Number
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53203
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Abstract
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Polyplexes between a double-stranded Salmon DNA and hyperbranched poly(ethyleneimine) (PEI) as well as a maltosylated PEI-Mal were incorporated into a gelatin/chitosan hydrogel scaffold. Calorimetric experiments of the polyplexes show a decrease of the melting temperature in presence of PEI and a peak splitting in presence of PEI-Mal, which can be interpreted to a partial compaction of the DNA strands in presence of PEI-Mal. When the polyplexes are incorporated into a gelatin/chitosan scaffold in the swollen state, the DNA melting peaks at 90 and 93 °C, respectively, indicate in both cases the release of the DNA at the surface of the hydrogel scaffold in a more compact form. Specific interactions between the PEI-Mal shell and gelatin are responsible for the tuning of the release properties in presence of the maltose units in the hyperbranched PEI.
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Publisher
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Composite Interfaces
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Wikidata
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Citation
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Composite Interfaces (2017) p.1-11
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DOI
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https://doi.org/10.1080/09276440.2017.1302725
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Tags
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dna-pei polyplexes maltosylated poly(ethyleneimine) µ-dsc dna release gelatin/chitosan hydrogel scaffold
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