Authors
|
Henke, S. ; Leijten, J. ; Kemna, E. ; Neubauer, M. ; Fery, A. ; van den Berg, A. ; van Apeldoorn, A. ; Karperien, M.
|
Title
|
Enzymatic crosslinking of polymer conjugates is superior over ionic or UV crosslinking for the on-chip production of cell-laden microgels
|
Date
|
01.10.2016
|
Number
|
50961
|
Abstract
|
Cell-laden micrometer-sized hydrogels (microgels) hold great promise for improving high throughput ex-vivo drug screening and engineering biomimetic tissues. Microfluidics is a powerful tool to produce microgels. However, only a limited amount of biomaterials have been reported to be compatible with on-chip microgel formation. Moreover, these biomaterials are often associated with mechanical instability, cytotoxicity, and cellular senescence. To resolve this challenge, dextran-tyramine has been explored as a novel biomaterial for on-chip microgel formation. In particular, dextran-tyramine is compared with two commonly used biomaterials, namely, polyethylene-glycol diacrylate (PEGDA) and alginate, which crosslink through enzymatic reaction, UV polymerization, and ionic interaction, respectively. Human mesenchymal stem cells (hMSCs) encapsulated in dextran-tyramine microgels demonstrate significantly higher (95%) survival as compared to alginate (81%) and PEGDA (69%). Long-term cell cultures demonstrate that hMSCs in PEGDA microgels become senescent after 7 d. Alginate microgels dissolve within 7 d due to Ca2+ loss. In contrast, dextran-tyramine based microgels remain stable, sustain hMSCs metabolic activity, and permit for single-cell level analysis for at least 28 d of culture. In conclusion, enzymatically crosslinking dextran-tyramine conjugates represent a novel biomaterial class for the on-chip production of cell-laden microgels, which possesses unique advantages as compared to the commonly used UV and ionic crosslinking biomaterials.
|
Publisher
|
Macromolecular Bioscience
|
Wikidata
|
|
Citation
|
Macromolecular Bioscience 16 (2016) 1524-1532
|
DOI
|
https://doi.org/10.1002/mabi.201600174
|
Tags
|
biomaterials enzymatic hydrogel microfluidic microgels mesenchymal stem-cells in-vitro high-throughput matrix elasticity hydrogels cartilage chondrocytes dextran dedifferentiation chondrogenesis
|