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Authors
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Theato, P. ; Zentel, R. ; Schwarz, S.
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Title
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Synthesis of end-functionalized lipopolymers and their characterization with regard to polymer-supported lipid membranes
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Date
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31.12.2002
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Number
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28742
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Abstract
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In order to build up tether-supported membranes, we have focused on the synthesis of lipopolymers. Different lipid analog initiators based on 2-bromopropionamide have been synthesized. In addition, fluorescence-labeled lipoinitiators using pyrene were synthesized. These initiators were used for the synthesis of polyacrylamide copolymers by atom-transfer radical polymerization. A final surface-anchor end group was attached to the polymers by polymer analogous reaction. In this way, a,ß-functionalized polyacrylamides were obtained. The interaction of these lipopolymers with different lipid bilayer structures was investigated in several experiments. The lipopolymers adsorb onto the surface of vesicles as shown by photon correlation spectroscopy and fluorescence measurements. The fixation of these lipopolymers on flat surfaces was studied using surface plasmon spectroscopy. The film thickness of the adsorbed lipopolymer films is about 12 to 20 Å. The surfaces thus modified can be used for the fixation of lipid vesicles to form polymer-supported bilayers. This leads to an additional thickness increase of 4162 Å.
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Publisher
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Macromolecular Bioscience
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Wikidata
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Citation
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Macromolecular Bioscience 2 (2002) 387-394
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DOI
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https://doi.org/10.1002/1616-5195(200211)2:8<387::AID-MABI387>3.0.CO;2-5
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