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Authors
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Zieris, A. ; Prokoph, S. ; Welzel, P. ; Grimmer, M. ; Levental, K. ; Panyanuwat, W. ; Freudenberg, U. ; Werner, C.
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Title
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Analytical approaches to uptake and release of hydrogel-associated FGF-2
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Date
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26.01.2010
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Number
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21004
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Abstract
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Strategies to control the delivery of growth factors are critically important in the design of advanced biomaterials. In this study we investigated the binding and release of fibroblast growth factor 2 (FGF-2) to/from a biohybrid hydrogel matrix by four independent analytical methods: radioisotope and fluorescence labeling, amino acid analysis and Enzyme-Linked Immunosorbent Assays (ELISA). The compared analyses provided qualitatively similar uptake characteristics while the results of the FGF-2 quantification strongly depended on the particular experimental conditions. The release kinetics of FGF-2 from the gels could be monitored sensitively by 125I labeling and by ELISA-techniques. The latter method was concluded to be advantageous since it permits the application of unmodified (native) growth factors.
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Publisher
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Journal of Materials Science : Materials in Medicine
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Wikidata
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Citation
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Journal of Materials Science : Materials in Medicine 21 (2010) 915-923
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DOI
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https://doi.org/10.1007/s10856-009-3913-z
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Tags
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MBC_Hydrogels
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