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Authors
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Himmel, H.M. ; Pietsch, M. ; Streller, U. ; Graf, E.M. ; Ravens, U.
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Title
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Changes in morphology and inward rectifier currents in human atrial myocytes depend on culture conditions
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Date
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31.12.2002
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Number
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10042
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Abstract
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Human atrial myocytes were cultured under systematically varied conditions in order to obtain stable cells for future gene manipulation. Transient (Ito) and sustained outward current (Iso), and voltage- and muscarinic receptor-activated inward rectifier K+ currents (IK1, IK,ACh) were measured in freshly isolated cells and after 5 days in culture. Myocytes were grown on polylysin or laminin in medium with or without 10 % serum (medium+S, medium-S). Cultured myocytes dedifferentiated to a greater extent in medium+S than medium-S, but independent of the chemical nature of the adherence surface. Apparent surface area increased in medium+S, whereas membrane capacitance declined under all culture conditions. Ito of myocytes cultured in medium-S was increased. Myocytes grown on polylysin and laminin exhibited reduced IK1 current density. Under all culture conditions, IK,ACh was attenuated with carbachol but hardly affected with sphingosine-1-phosphate as agonists. In conclusion, morphological and electrophysiological changes depended on serum in the culture medium rather than on adherence surface being coated with laminin or polylysin.
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Publisher
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Basic Research in Cardiology
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Wikidata
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Citation
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Basic Research in Cardiology 97 (2002) 434-444
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DOI
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https://doi.org/10.1007/s00395-002-0375-6
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