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Title Ni(II)-NTA modified poly(ethylene imine) glycopolymers: Physicochemical properties and first in vitro study of polyplexes formed with HIV-derived peptides
Date 11.03.2013
Number 37562
Abstract Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)-nitrilo(triacetic acid)-modified poly(ethylene imine)-maltose (Ni-NTA-DG) is realized and evaluated as complexation agent against His-tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5–6.0, and also up to 50?mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte-derived immature dendritic cells. In summary, Ni-NTA-DG represents a promising (delivery) platform for forthcoming in vitro applications.
Publisher Macromolecular Bioscience
Identifier
Citation Macromolecular Bioscience 13 (2013) 531-538
DOI https://doi.org/10.1002/mabi.201200449
Authors Hauptmann, N. ; Pion, M. ; Munoz Fernández, M.-Á. ; Komber, H. ; Werner, C. ; Voit, B. ; Appelhans, D.
Tags dendritic cells dendritic glycopolymers nta ligands poly(ethylene imine) polyplexes dendritic cells tagged proteins hyperbranched-pei purification delivery immunotherapy complexes dna

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