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Title A practical guide to quantify cell adhesion using single-cell force spectroscopy
Date 20.02.2013
Number 36184
Abstract Quantitative analysis of cellular interactions with the extracellular environment is necessary to gain an understanding of how cells regulate adhesion in the development and maintenance of multicellular organisms, and how changes in cell adhesion contribute to diseases. We provide a practical guide to quantify the adhesive strength of living animal cells to various substrates using atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS). We describe how to control cell state and attachment to the AFM cantilever, how to functionalize supports for SCFS measurements, how to conduct cell adhesion measurements, and how to analyze and interpret the recorded SCFS data. This guide is intended to assist newcomers in the field to perform AFM-based SCFS measurements.
Publisher Methods : a companion to methods in enzymology
Identifier
Citation Methods : a companion to methods in enzymology 60 (2013) 169-178
DOI https://doi.org/10.1016/j.ymeth.2013.01.006
Authors Friedrichs, J. ; Legate, K. R. ; Schubert, R. ; Bharadwaj, M. ; Werner, C. ; Müller, D. J. ; Benoit, M.
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