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Authors Kokesch-Himmelreich, J. ; Woltmann, B. ; Torger, B. ; Rohnke, M. ; Arnhold, S. ; Hempel, U. ; Müller, M. ; Janek, J.
Title Detection of organic nanoparticles in human bone marrow-derived stromal cells using ToF-SIMS and PCA
Date 01.06.2015
Number 46025
Abstract The detection and localization of polymer-based nanoparticles in human bone marrow-derived stromal cells (hBMSC) by time-of-flight secondary ion mass spectrometry (ToF–SIMS) is reported as an example for the mass spectrometry imaging of organic nanoparticles in cell environments. Polyelectrolyte complex (PEC) nanoparticles (NP) made of polyethylenimine (PEI) and cellulose sulfate (CS), which were developed as potential drug carrier and coatings for implant materials, were chosen for the imaging experiments. To investigate whether the PEI/CS–NP were taken up by the hBMSC ToF–SIMS measurements on cross sections of the cells and depth profiling of whole, single cells were carried out. Since the mass spectra of the PEI/CS nanoparticles are close to the mass spectra of the cells principal component analysis (PCA) was performed to get specific masses of the PEI/CS–NP. Mass fragments originating from the NP compounds especially from cellulose sulfate could be used to unequivocally detect and image the PEI/CS–NP inside the hBMSC. The findings were confirmed by light and transmission electron microscopy.
Publisher Analytical and Bioanalytical Chemistry
Wikidata
Citation Analytical and Bioanalytical Chemistry 407 (2015) 4555-4565
DOI https://doi.org/10.1007/s00216-015-8647-9
Tags mass spectrometry imaging organic nanoparticles single cell analysis tof–sims human bone marrow-derived stromal cells internalization

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