|
Authors
|
Korn, P. ; Elschner, C. ; Hautstock, M. ; Range, U. ; Pradel, W. ; Lauer, G.
|
|
Title
|
Method comparison between bone histology and magnetic resonance imaging: is magnetic resonance imaging suitable to quantify osteogenesis?
|
|
Date
|
01.03.2017
|
|
Number
|
53177
|
|
Abstract
|
Currently, histological techniques are mainly used to analyse biomaterial-tissue-interactions. However, these methods are destructive and time-consuming. Furthermore, they require a large number of animals as longitudinal observations are not possible. The evaluation by non-destructive imaging techniques like magnetic resonance imaging (MRI) could be an alternative, but is has to be evaluated if the new methods enable also sufficient quantification, e.g. of bone formation. The present study examined the suitability of MRI to assess osteogenesis in an artificial maxillary bone defect in a rat model. The bone defects (n = 84) were augmented with 3 different bone grafts, as well as an empty control defect was analysed. After 6, 9 and 12 weeks the bony ingrowth and the remaining defect width were measured by histomorphometry and MRI. By using a graphical concordance analysis the mean difference between MRI and histological results was clearly detectable. For the parameter bone volume the histomorphometrical result was on average 2.4% higher than the MRI values. For the parameter remaining defect width there was also a statistical significant bias between both methods measurable (6.7%). Reason for the data bias is mainly the analysed section position, which could be proven in a repeated measurement experiment.<br />In conclusion the application of MRI enables sufficient defect visualisation, but the quantitative results differed in comparison with histology while not exactly the same slice positions were analysed. If the same slice is analysed by both methods the results are absolutely comparable and the MRI is suitable to assess osteogenesis.
|
|
Publisher
|
International Journal of Oral and Maxillofacial Surgery
|
|
Wikidata
|
|
|
Citation
|
International Journal of Oral and Maxillofacial Surgery 46 (2017) Suppl. 1, 208
|
|
DOI
|
https://doi.org/10.1016/j.ijom.2017.02.707
|
|
Tags
|
|
