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Authors
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Polikarpov, N. ; Potolytsyna, V. ; Bessonov, E. ; Tripp, S. ; Appelhans, D. ; Voit, B. ; Kartsova, L.
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Title
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Dentritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: View on protein separation processes and detection of nanogram-scaled albumin in biological samples
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Date
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23.01.2015
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Number
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42483
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Abstract
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Unique properties of dendritic polymers make them promising candidates for application as additives in various analytical methods. From this point, we investigated the potential use of maltose-modified hyperbranched poly(ethylene imine) (PEI-Mal) as a dynamically or covalently bound coating and a pseudostationary phase in capillary electrophoresis. The EOF mobilities were measured at different pH values (2.2, 8.5, and 10.2) using PEI-Mal in the background electrolyte (BGE) and desirable repeatability of the EOF with % RSD (n = 50) =3.3 was obtained. The influence of pH, polymer concentration, and density of maltose shell on the separation properties of a model mixture of four proteins (albumin, lysozyme, myoglobin, insulin) were investigated. Applying PEI-Mal as a dynamic coating, improved separation of the protein mixture with a high repeatability was achieved. Applying PEI-Mal as a covalent coating for concentrating proteins in the large volume sample stacking (LVSS) combined with the field-enhanced sample injection (FESI), up to 1320-fold enhancement of sensitivity was achieved. The detection limit of 100–500 ng/ml allowed successful analysis of albumin level both in blood and urine samples without additional preconcentration.
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Publisher
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Journal of Chromatography / A
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Wikidata
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Citation
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Journal of Chromatography / A 1378 (2015) 65-73
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DOI
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https://doi.org/10.1016/j.chroma.2014.11.051
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Tags
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hyperbranched polymers proteins capillary electrophoresis dynamic coating covalent coating on-line concentration poly(propylene imine) dendrimers stationary phases quaternized celluloses hydroxyethylcellulose complexation ce chromatography performance col
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