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Authors
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Bucatariu, F. ; Ghiorghita, C.-A. ; Mihai, M. ; Simon, F. ; Dragan, E. S.
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Title
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Pepsin and lysozyme immobilization onto daisogel particles functionalized with chitosan cross-linked multilayers
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Date
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21.05.2013
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Number
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38163
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Abstract
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One type of cross-linked multilayer, based on chitosan (CHI) has been prepared using 3,3,4,4-benzophenonetetracarboxylic-dianhydride (BTCDA) and glutaraldehyde (GA) as cross-linkers. CHI was adsorbed from acetic acid aqueous solution onto Daisogel microparticles. X-ray photoelectron spectroscopy showed a regular increase of the cross-linked multilayers onto Daisogel microparticles. The immobilization of pepsin and lysozyme onto the functionalized Daisogel surface, via GA, has been tested. The amount of immobilized enzymes significantly depended on isoelectric point of enzyme.
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Publisher
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Revista de chimie
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Wikidata
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Citation
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Revista de chimie 64 (2013) 334-337
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DOI
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http://www.revistadechimie.ro/article_ro.asp?ID=3637
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Tags
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