Menü

Publikationsliste

Authors Bucatariu, F. ; Simon, F. ; Fundueanu, G. ; Dragan, E. S.
Title Design of silica microparticles with oligopeptide brushes and their interaction with proteins
Date 31.01.2011
Number 25110
Abstract Synthesis of small oligopeptide brushes (oligo(Sbenzyl-L-cysteine)) onto polyelectrolyte functionalized silica microparticles was developed. Poly(vinyl amine) (PVAm) adsorbed from salt-free and KCl 10-1 mol L-1 aqueous solution onto silica microparticles was chemically and naturally cross-linked by epichlorohydrin and CO2, respectively. After the adsorption of PVAm onto microporous silica particles and stabilization by cross-linking, five repeated coupling reactions of Boc-S-benzyl-L-cysteine were performed. To test the protein interactions with the newly designed surface, human serum albumin (HSA) has been selected as a model protein. X-ray photoelectron spectroscopy, total organic carbon, potentiometric and polyelectrolyte titrations, and electrokinetic analysis were employed to obtain information about the polyelectrolyte adsorption and the amount of the amino acid S-benzyl-L-cysteine that was covalently bound to the solid surface and for determination of the protein amount adsorbed onto functionalized surface.<br /><br />The amount of HSA adsorbed onto modified silica microparticles decreased in order: silica/PVAm-cross-linked (silica/ PVAm-C) (8.00 mg g-1) > silica/PVAm-C/S-benzyl-L-cysteine (6.34 mg g-1) > silica (4.86 mg g-1) > silica/PVAm-C/(Sbenzyl-L-cysteine) (1.86 mg g-1).<br />
Publisher Colloid and Polymer Science
Wikidata
Citation Colloid and Polymer Science 289 (2011) 33-41
DOI https://doi.org/10.1007/s00396-010-2319-2
Tags

Back to list