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Uhlmann, P. ; Bittrich, E. ; Kempe, F. ; Stamm, M.
Switching of protein adsorption on polyelectrolyte brushes

The generation of thin polymer layers offering defined interaction to proteins is of unique interest in biomaterial research and development and still a challenging scientific topic. Both attractive interaction as well as repulsive one and especially switching between both interaction states are greatly relevant. Polymer brushes are a versatile tool to control and switch interfacial properties forming very thin reproducible and stable polymer layers at various substrates. By composing polymer brushes of two or more specific polymers/ polyelectrolytes a big variety of possibilities arises to control the interaction of those surfaces with proteins by controlling e.g. hydrophilic/hydrophobic balance and "pachiness", surface charge composition, morphology etc. Using polymers responsive to environmental conditions as pH or temperature, the interfacial properties of mixed brushes may be switched. For using this possibility to switch protein adsorption too, it is essential to study mechanism and kinetics of protein adsorption and desorption on those polyelectrolyte containing brushes in detail to identify the leverage for an adsorption/desorption control. We studied the influence of electrostatic interactions, hydrophobic interactions, entropic driving forces etc. on the adsorption of a set of model proteins by changing brush composition and environmental conditions by using in-situ-ellipsometry, ATR-FTIR spectroscopy as well as AFM. We discuss our results before the backround of current theories of protein adsorption and name some leverages for its control.

Quelle
Polymeric Materials: Science and Engineering 99

Seiten
118-119

DOI
http://oasys2.confex.com/acs/236nm/techprogram/P1201742.HTM

Erschienen am
September 2008
 
Publications
Publications

Departments

Nanostructured Materials