Layman, J. ; Appelhans, D. ; Voit, B. ; Long, T. E.
Oligosaccharide-modified polymers as nucleic acid transfection agents

POLY 218 - Synthetic and Biological Macromolecules for Emerging Nanotechnologies

Non-viral gene transfection agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular architecture. Although non-viral vectors possess numerous design advantages, several investigators have shown that transfer efficiencies are considerably lower when compared to viral vectors. In order to more fundamentally understand structure-transfection relationships, our laboratories are currently focused on evaluating the influence of various oligosaccharide modifications on the cytotoxicity and transfection activity of hyperbranched poly(ethyleneimine) (PEI). In this study, the oligosaccharide modification was found to reduce cell cytotoxicity; however, the modification was also found to eliminate the polymer's ability to transfect DNA.

Quelle
Polymer Preprints 48

Seiten
952-953

DOI
http://oasys2.confex.com/acs/234nm/techprogram/P1118232.HTM

Erschienen am
August 2007