Blood Activation Processes

Mission

…is the definition of a hemocompatible profile for biomaterial surfaces leading to knowledge based design of hemocompatible material surfaces.

Biomaterials directly contacting human blood induce reactions of blood proteins and cells. The intensity and type of these reactions depend on biomaterials’ surface characteristics like roughness, charge, hydrophilicity and domain structure. There are numerous hypothesises on initial events of the blood-materials interaction yet due to the complexity of the reactions lots of questions remain unanswered. The analysis of interactions of materials with blood components relies on the use of a realistic incubation set-up combined with well defined parameters as well as surfaces which enable a direct correlation between reactions and surface characteristics.

Our custom made incubation systems enable the incubation of a variety of material types with fresh whole human blood under flowing or non-flowing conditions. The analysis of reactions of the incubated blood and the corresponding surfaces encompasses a wide range of analytical methods. Additionally several methods following surface incubation with different blood fractions like blood plasma, cells or proteins aid in the trial to get a whole picture of blood surface interactions. The incubated surfaces are well defined models like self-assembled monolayer (SAM) exposing a variety of different functional groups and combinations of the latter to scrutinize fundamental hypothesises on initial events of the blood-materials interaction. Additionally polymer films using poly(octadecene (or alternatively ethylene- or propylene-) alt maleic anhydride) copolymers are used.

Group Members

Grants

  • Initiation of blood coagulation – Evaluating the relevance of specific surface functionalities using self assembled monolayer (DFG SP 966/2-2)

Selected Publications

  1. Werner, C.; Maitz, M.F.; Sperling, C.
    Current strategies towards hemocompatible coatings mehr
    Journal of Materials Chemistry 17 (2007) 3376-3384

  2. Sperling, C.; Maitz, M.F.; Talkenberger, S.; Gouzy, M.-F.; Groth, T.; Werner, C.
    In vitro blood reactivity to hydroxylated and non-hydroxylated polymer surfaces mehr
    Biomaterials 28 (2007) 3617-3625

  3. Sperling, C.; Schweiß, R.; Streller, U.; Werner, C.
    In vitro hemocompatibility of self-assembled monolayers displaying various functional groups mehr
    Biomaterials 26 (2005) 6547-6557

  4. Streller, U.; Sperling, C.; Hübner, J.; Hanke, R.; Werner, C.
    Design and Evaluation of Novel Blood Incubation Systems for In Vitro Hemocompatibility Assesment of Planar Solid Surfaces mehr
    Journal of Biomedical Materials Research 66b (2003) 379-390

Oral presentations

  1. Sperling, C. ; Schweiss, R. ; Werner, C. : Initiation of blood coagulation - evaluating the relevance of specific surface functionalities using self assembled monolayers. - 21th European Conference on Biomaterials. - Brighton, UK, 09.09.2007 - 12.09.2007
  2. Sperling, C. ; Fischer, M. : Coagulation activation on SAM model surfaces with varied composition of hydrophobic and negatively charged alkanethiols. - INEB - Instituto Nacional Engenharia Biomédica. - Porto, Portugal, 16.04.2008 - 20.04.2008
  3. Sperling, C. ; Maitz, M.F. ; Talkenberger, S. ; Gouzy, M.-F. ; Groth, T. ; Werner, C. : Complement activation and granulocyte adhesion on model surfaces bearing various hydroxylic groups. - 8th World Biomaterials Congress. - Amsterdam, 28.05.2008 - 01.06.2008
 
Blood Activation Processes
Blood Activation Processes
 

Departments

Institute of Biofunctional Polymer Materials

Fields of Work

Hemocompatible Interfaces

Blood Activation Processes